Determining the properties of an enzyme lab report

How the changes in substrate concentration (PNPP), changes in temperature, changes in pH and the presence of an inhibitor (phosphate ions) effects the rate of reaction of the reaction between PNPP and water catalysed by the enzyme acid phosphates which produces PNP and phosphate as its products.However after the concentration increase from 0.20- to 3.00 Mmols the initial velocity shows a gradual increase and then gradually levels of or slopes here graph shows 0 order relationship.

Enzyme Kinetics - University Science Books - Home

A possible explanation for the change in the experimental design is that the pH may denature the enzyme if the enzyme was added first the active site and 3 dimensional shape of the enzyme could be altered at extremes of pH such as 2 which is quite acidic.Our Marking Service will help you pick out the areas of your work that need improvement.All procedures were carried out as described in the lab schedule.

Enzyme Kinetics (2006) - NC State Biochemistry

The optimum temperature of the reaction was in the range 310 K- 323.2 where the rate increased at its fastest.

Investigating Enzyme Activity Under a Variety of Conditions

Collected data are compiled in tables and graphs for use in a lab report. Lab Topic 5 Determining the Properties of an.Objective: To determine the optimal. section of your lab report.Abstract Enzymes are responsible for the speed at which chemical.An in vitro reaction was performed to examine the catalytic properties of alkaline phosphatase (ALP) a hydrolase enzyme and the synthetic substrate 4-nitrophenyl phosphate (4-NPP).

The product produced is yellow and can be measured using a colorimeter.The relationship between the rate of an enzyme-catalysed reaction and the substrate concentration can be describes as hyperbolic.A clean cuvette containing water was used to zero the spectrophotometer and set aside to be used before each reading.

Introduction to Enzymes. published in 1972 as the Manual of Clinical Enzyme. the properties of enzymes that makes them so important as.My findings support the findings of many previous investigations and journals as the graphs show a similar relationship as to the findings to previous work.The enzyme catalase is responsible for speeding up. on catalase activity.The graph and tables 2 show that as the concentration of substrate (PNPP) increases from 0.00 Mmols to 0.20 Mmols of substrate the initial velocity or the rate of reaction shows an increase in too.As previously, the enzyme was added last (except in the case of the cuvette containing no enzyme) and stirred thoroughly before being measured for absorptivity using a blank cuvette to zero the spectrophotometer and results recorded over a 25 min period in 5 min increments.The initial absorbance of the 4-NP increased linearly to a high degree for a short time before dropping off and decreasing, whilst the 4-NPP steadily decreased in absorptivity(Fig 1).I predicted an increase in temperature will increase the rate of reaction or the initial velocity of the e reaction until the enzyme denatures and the protein sequence becomes damaged an unravels.

According to the induced fit hypothesis the enzyme can undergo some change in the 3- dimensional structure in order to fit the substrate.

Laboratory Inquiry 1: Enzymes and Enzymatic Reactions

Enzyme Lab Report Of Alkaline Phosphatase Biology Essay. product 4-NP to determine the spectral properties of.

Specificity of Enzymes (Introduction to Enzymes)

No alterations were made to any of the procedures carried out throughout the experiment.

Biology 100 Lab Report: “pH and Its Affect on Enzymes

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Biology Lab Report Example On Enzymes

This is an exercise in which the properties of enzymes are investigated.This feature is important for it enables determination of the amount of enzyme in an unknown sample.Lectures notes (2009) Dr John Mills- Module LSC-10034 Proteins and Enzymes - lectures 9, 10, 11 (Figures 1-5).


However it is important to mention that at high substrate concentration the enzyme will be fully saturated this will reduce the rate of reaction as the there are too many substrate than enzymes.There is an increase in the rate of reaction as the temperature increases from 278 -323 K the increase is almost linear.This would mean that no reaction can be completed so the rate of reaction will decrease.The purpose of this scientific paper was to replicate earlier findings of experiments in enzyme kinetics and to see if enzyme behaviour and activity is influenced by 4 factors- change in substrate concentration, temperature, pH and the presence of a competitive inhibitor.Non competitive inhibitor has the same effect as lowering the total volume of enzyme.

Section B of this investigation concerns the effect of temperature on the rate of reaction.This is not an example of the work written by our professional essay writers.

BIOCHEMISTRY: Experiment 3 : Enzyme Kinetics

Get help with your Vancouver referencing with our free online tool.The next section of the investigation concerns the effect of temperature on the rate of reaction.